Coding

Part:BBa_K129005

Designed by: Burak YILMAZ   Group: iGEM08_METU_Turkey   (2008-11-08)


LamB_CP-metal binding domain in LamB-153 residue

LamB tolerates insertions of long heterologous peptides at a permissive loop (between structural codons 153 and 154) exposed to the external medium without a loss of function.CP (Gly-Cys-Gly-Cys- Pro-Cys-Gly-Cys-Gly), employed in LamB and called LamB_CP. Cp was previously found as cysteine and histidine residues for Cd21 binding (29). CP was further characterized and employed for display on the E. coli surface.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 1325
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal SpeI site found at 1326
    Illegal PstI site found at 1340
    Illegal NotI site found at 7
    Illegal NotI site found at 1333
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 1326
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal XbaI site found at 16
    Illegal SpeI site found at 1326
    Illegal PstI site found at 1340
    Illegal AgeI site found at 98
    Illegal AgeI site found at 1202
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None